Maithani Meters et ing. / Pharmacie Globale (IJCP) 2010, 5 (05)
Available at www.pharmacie-globale.info
INTERNATIONAL JOURNAL OF COMPREHENSIVE PHARMACY
DEVELOPMENT AND AFFIRMATION OF A RP-HPLC METHOD FOR THE DETERMINATION OF CHLORPHENIRAMINE MALEATE AND PHENYLEPHRINE IN PHARMACEUTICAL DRUG DOSAGE TYPE
Mukesh Maithani*, Richa Raturi, Vertika Gautam, Dharmendra Kumar, Amrendra Kumar Chaudhary, Anand Gaurav and Ranjit Singh
School of Pharmaceutical Sciences, Shobhit School, NH-58, Modipuram, Meerut, Uttar Pradesh (250 110), India. Received: 6th November 2010; Revised: 30 November 2010; Accepted: on the lookout for December 2010; Available online: doze December 2010
A straightforward, specific and accurate invert phase powerful liquid chromatographic method was created for the simultaneous willpower of chlorpheniramine maleate and phenylephrine in tablet dose forms. A reversed-phase C-18 column (250 mm Г— 8 logistik i. m., particle size 10 Вµm) column with mobile stage consisting of acetonitrile and phosphate buffer fifty five: 45 (v/v) (pH five. 6 В± 0. 02, adjusted with triethylamine) was used. The flow rate was 1 . 0 ml/ min and effluents were watched at 255 nm. The retention times of chlorpheniramine maleate and phenylephrine were found to be three or more. 13 min and some. 58 minutes, respectively. The strategy was authenticated in terms of linearity, range, specificity, accuracy, accurate, limit of detection (LOD) and limit of quantitation (LOQ). The linearity for the drugs was found in kids of 10-70 Ојg/ml. The % recoveries of chlorpheniramine maleate and phenylephrine were found being between 101. 09 and 98. 99. The suggested method was successfully applied to the estimation of chlorpheniramine maleate and phenylephrine in combined tablet dosage forms. Keywords: Chlorpheniramine maleate, phenylephrine, simultaneous appraisal, RP-HPLC, tablet dosage forms.
Chlorpheniramine maleate (CM) chemically, 3-(4chlorophenyl)-N, N-dimethyl-3-pyridin-2-ylpropan-1amine is usually an antihistamine drug that may be widely used in pharmaceutical arrangements for symptomatic relief of
common chilly and sensitive diseases. Phenylephrine (PE)
ethanol hydrochloride can be used as a sympathomimetic. 1-4
The structures of CM and PE happen to be shown in (Figure 1).
Numerous ULTRAVIOLET, HPLC and HPTLC centered methods have already been
reported pertaining to estimation of these drugs exclusively as well as in
blend with other medicines in pharmaceutic dosage
forms. 5-14 Yet no technique had but been reported for
sychronizeds estimation of such two medications using HPLC in
large drug and pharmaceutical serving forms. Therefore ,
the present job was was executed to develop and validate a fresh
RP- HPLC method for coexisting estimation of CM and
PE in pharmaceutical serving forms.
Number 1 . The structures of chlorpheniramine maleate
(CM) and phenylephrine (PE)
Chlorpheniramine maleate (CM)
Lecturer, School of Pharmaceutical drug Sciences,
Shobhit University, NH-58, Modipuram,
Meerut, Uttar Pradesh- 250 110, India.
Contact no: +91-9758860810
Email: [email protected] com
C CH2NHCH3. HCl
MATERIALS AND METHODS
Chemicals and Reactants
Reference standards of CM and PREMATURE EJACULATION RAPID EJACULATION, RAPID CLIMAX, PREMATURE CLIMAX, were procured as gift
samples by Torrent Pharmaceutical (Gandhinagar,
India). HPLC class acetonitrile, normal water and triethylamine
were from Rankem, RFCL Limited, Fresh Delhi,
India. Potassium dihydrogen orthophosphate AREAL and
ortho phosphoric chemical p AR grade were procured from
Central Drug Residence (P) Limited, New Delhi, India.
The HPLC (PerkinElmer series 200) instrument equipped
having a model series 200 pump, vacuum degasser,
rheodyne injector with a 20Ојl loop, UV-Visible detector
and C-18 column was used.
The isocratic cellular phase was consisted of...
Recommendations: 2 . British Pharmacopoeia, The Stationary Workplace: London,
4. Current Index of Medical Specialties, Medimedia Health
Non-public Ltd.: Bangalore, 2006, 253.